Figure 7. Response of paired box gene 6 (PAX6) knockdown on rhodopsin (RHO) expression inretinoblastoma cell lines. PAX6 siRNA was introduced into Y-79 and WERI cells via siPORT for knockdown of PAX6 mRNA. The electrophoresis photographs and the bar graphs indicate the result of reverse transcription polymerase chain reaction (RT–PCR) in Y-79 cells (A) and WERI cells (B). PAX6 and RHO mRNA expression were investigated using RT–PCR. The mRNA expression was detected 48 h after siRNA transfection and cotylenin A (CN-A) treatment. The bar graph indicates transduction of PAX6, RHO, and GAPDH mRNA expression by PAX6 siRNA (n=3); the error bar indicates the standard deviation; Mann–Whitney U-test, *p<0.05, **p<0.01.