Figure 5. Luciferase assay of paired box gene 6 (PAX6) promoter activity. A: Schematic indicates the representation of the PAX6 transcript unit in humans. The boxes denote exons. Braces indicate the extent of P0, P1, and Pα promoters in the PAX6 gene area. B: Luciferase assay of P0, P1, and Pα promoter activity in Y-79 cells. C: Luciferase assay of P0, P1, and Pα promoter activity in WERI cells. P0 of pGL4.10 insert indicates the insertion of P0 promoter sequences (5315 bp), P1 promoter activity (3796 bp), and Pα promoter sequences (3628 bp). White and black squares indicate the no-CN-A treatment and CN-A treatment, respectively. The negative pGL4.10 insert indicates an empty sequence. The data are presented after normalizing transfection efficiency using the Renilla luciferase reporter gene (n=3); the error bar indicates the standard deviation; Mann–Whitney U-test, *p<0.05.