Figure 4. Expression of mRNA and protein in retinoblastoma cells. A: Some gene mRNA expression was investigated using reverse transcription polymerase chain reaction (RT–PCR). Cells were treated with or without 10 μg/ml cotylenin A (CN-A) for 3 days. The numbers (0, 10, and 20) indicate CN-A concentrations (μg/ml). β-Actin mRNA was used as an internal control. B: Some protein expression was investigated using western blot analysis in retinoblastoma cells. Cells were treated with or without 10 μg/ml CN-A for 5 days. “Cont.” indicates the non-treatment. “CN-A” indicates the CN-A treatment (10 μg/ml). β-Tubulin protein was used as a loading control. C: The bar graph indicates the PAX6 protein expression (n=3); the error bar indicates the standard deviation.