Figure 6. Analysis of dissociated chick retina indicates that 2M6 is a Müller cell-specific protein associated with internal membranes. Monolayer cultures from chick retina were prepared, fixed, and stained with antibodies specific for 2M6 (D, red) and human natural killer cell antigen (HNK; C, green), and with 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5; B, blue). The overlay image (A) shows that 2M6-expressing and HNK-expressing cells are distinct from one another. No overlap of expression was observed, which indicates that 2M6 is not expressed by HNK-expressing neurons. Panel E is a computer-generated “side view” of the 2M6 signal showing that the reticulations within the Müller cell are distributed throughout the depth of the cell. A differential interference contrast image is also shown in panel B. The magnification bars for A–D are 18.11 μm; the magnification bar for E is 18.34 μm.