Figure 1. Immunoblotting analysis of 2M6
antigen purified from chick retina. Proteins obtained from embryonic
day 13 chick retina were subjected to affinity purification using the
2M6-specific antibody conjugated to cyanogen bromide-conjugated
(CNBr)-activated sepharose 4B. The eluate was subjected to SDS–PAGE and
subsequent immunoblotting analysis using the 2M6-specific antibody.
Signals were observed for polypeptides at ~40 kDa, ~46 kDa,
and ~80 kDa. These polypeptides were subjected to in-gel
trypsinization and subsequent mass spectrometry analyses. Lanes 1 and 2
show duplicate samples of the eluted fraction with the highest
absorbance measured at 280 nm.