Figure 8. A 3D reconstruction of a TM region from Figure 7. The area of TM shown in Figure 7 that encompasses the large ‘fluid space’ near the center was imaged at 0.5 µm intervals. A total of 85 z-sections were projected into a 3D-animation, with the 2PEF from the Hoechst-labeled nuclei shown in blue and the SHG from collagen in white. The animation begins with a view of the aqueous face of the TM, with rotation about the y-axis. The pattern of blue-stained nuclei indicates that TM endothelial cells are evenly distributed throughout the collagen bundles of this region.

Note that the slide bar at the bottom of the quicktime movie can be used to manually control the flow of the movie, or double click on the image to automatically run the animation. If you are unable to view the movies, a representative frame from each movie is included below.