Molecular Vision: Semple-Rowland, Mol Vis 2010; 16:916-934. Table 1

Table 1. PCR primers for lentivirus vector constructs

Vector insert	Primer name	Sequence
RK
	S-BamHI	TTAGGATCCGGGCCCCAGAAGCCT
	S-EcoRV	TTAGATATCGGGCCCCAGAAGCCT
	AS- KpnI	ATTGGTACCGCCCTTGGCCTGTGG
	AS-PmeI	ATTGTTTAAACGCCCTTGGCCTGTGG
XOPS
	S-NheI	ATTGCGGCCGCAGATCTTTATACATTGCT
	AS-NheI	AATGCTAGCCTCGAGATCCCTAGAAGC
NRLL
	S-NotI	TTGCGGCCGCGCGCTACCGGACTCA
	AS-NheI	GCGCTAGCGGCGACCGGTGGAT
EFIα
	S-NotI	ATTGCGGCCGCTTTGGAGCTAA
	AS-NotI	TTAGCGGCCGCCACGACACCTGAAAT
WPRE
	S-SpeI	ATAACTAGTATAATCAACCTCTGGATT
	S-MluI	ATAACGCGTATAATCAACCTCTGGATT
	S-KpnI	GGTGGTACCATAATCAACCTCTGGATT
	AS-SpeI	ATAACTAGTGGTCGACGGTATCGATGC
	AS-MluI	ATAACGCGTGGTCGACGGTATCGATGC
	AS-KpnI	ATAGGTACCGGTCGACGGTATCGATGC
mCherry
	S-NheI	AAAGCTAGCAAGGATCCCGCCACC
	AS-NheI,MluI	AAAGCTAGCACGCGTCGTACGTCGGGCTTTGT
GFP
	S-NheI	GCAGCTAGCCGCCACCATGAGCAA
	AS-NheI, MluI	AATGCTAGCACGCGTCGTACGAGAGGCCTCAG
	S-XhoI	CCTCTCGAGCGCCACCATGAGCAA
	AS-XhoI	AATCTCGAGAGAGGCCTCAGTCAG
IRBPI56
	S-NheI	TTGCGGCCGCGCGCTACCGGACTCA
	AS-NotI	GCGCTAGCGGCGACCGGTGGAT
RK-GFP
	S-SpeI	ATACTAGTGGGCCCCAGAAGCCT
	AS-SpeI, MluI
	S-XhoI	ATCTCGAGGGGCCCCAGAAGCCT
	AS-XhoI	ATCTCGAGGATATCTTACTTGTACAGCTCGTCC
tdTomato
	S-PmeI	AATGATGTTTAAACCTGTACGACGATGACGATAAG
	AS-SalI	TTACATGTCGACTCCTTTCGGGCTTTGTTA
Vimentin
	S-MluI	GGCACGCGTCTGTTTTACCCACCATCTCAGTTCTAATATT
	AS-MluI	GGCACGCGTGGTACCCGGGGATCCACTAGTTCTAGAAAT