Figure 8 of Semple-Rowland, Mol Vis 2010; 16:916-934.

Figure 8. Expression characteristics of dual promoter vectors constructed using murine opsin promoter (MOPS) and Xenopus opsin promoter (XOPS) promoters. pFIN-XOPS-tdTOM-MOPS-GFP (1.6×108 vector genomes/µl; A-J) or pFIN-MOPS-GFP-XOPS-tdTOM-WPRE (4.4×108 vector genomes/µl; K-T) lentivirus was injected into the developing neural tubes of E2 chicken embryos in ovo. Retinal whole mounts (one retina per horizontal row) were photographed twice using the exposure duration shown in lower left of each panel and filters appropriate for detection of tdTOM or GFP. The merged images (C, H, M, R) were analyzed using the co-localization module of the Zeiss AxioVision Image Suite. The relative percent area (pixels) of each image containing GFP (green bar) or tdTOM (red bar) fluorescence alone or both GFP and tdTOM (yellow bar) is shown in panels D, I, N, S. The images shown in E, J, O, T were derived from the merged images (C, H, M, R) and show only those areas of the merged image in which GFP was co-localized with CHER. The scale bar shown in A is applicable to all images and equals 50 µm.