Figure 6. Down-regulation of proliferating cell nuclear antigen protein by mall interfering ribonucleic acid in rabbit lens epithelial cells in vitro. Immunofluorescence staining indicated the expression of PCNA decreased in rLECs transfected with Skp2 siRNA (C) when compared with rLECs transfected with pSuppressor vehicle (A) or without transfection (B). The scale bar is 40 μm. Western blot showed that the expression of PCNA dramatically decreased after rLEC transfection with Skp2 siRNA (D) when compared with rLEC transfection with the pSuppressor vehicle or without transfection. GAPDH served as the loading control.