Figure 5. S phase-kinase-interacting protein 2 protein by small interfering ribonucleic acid inhibited cell proliferation of rabbit len epithelial cells in vitro (3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide). For Brdu incorporation, cells growing on coverslips were incubated with Brdu. Incorporated Brdu was detected with antibodies as described in the Materials and Methods. High levels of BrdU with dense brown staining can be detected in rLEC of vehicle control (A) and blank control (B), but decreased in Skp2 siRNA transfectant cells (C). Statistical analysis (D) after cell counting showed that Brdu positive cells decreased after rLEC transfection with Skp2 siRNA compared with control cells. p<0.01 versus vehicle and blank control (n=32).