Figure 3 of Su, Mol Vis 2010; 16:907-915.


Figure 3. S phase-kinase-interacting protein 2 protein by small interfering ribonucleic acid induced p27 kinase inhibition protein 1in rabbit lens epithelial cells in vitro. Immunofluorescence staining indicated the expression of p27kip1 dramatically increased in rLECs transfected with Skp2 siRNA (C) when compared with rLECs transfected with pSuppressor vehicle (A) or without transfection (B). The scale bar is equal to 40 μm. Western blot (one representative of three experiments) showed that the expression of p27kip1 dramatically increased in rLECs transfected with Skp2 siRNA (D) when compared with rLECs transfected with pSuppressor vehicle (D) or without transfection (D). GAPDH served as the loading control.