Molecular Vision: Zangerl, Mol Vis 2009; 15:927-936. Table 1

Table 1. EST loci retained at each experimental step.

EST loci	Number	Percent
Tested	1,147	100.0
Amplified on dog DNA	998	87.0
Amplified satisfactorily on the RH5000–2 panel	711	62.0
Readable scores for all cell lines	555	48.4
Linked to at least one other marker	553	48.2
Mapped in unique position	501	43.7

To assess quality and efficiency of primer design, all primers were first amplified on dog DNA. Subsequently, working primer pairs were amplified on the RH panel, which proved to be the least efficient step. Of those satisfactorily amplifying on the panel, almost 80% could be scored in all cell lines, and only two of the scored ESTs did not provide sufficient linkage to at least on other marker. Finally, 90% of all linked markers were assigned to unique positions in the chromosome, thus yielding results for 44% of all tested primers without further optimization.