Figure 1. Suppression of TGFβ-induced lens opacification by GSH. A,B: Dark field microscopy. Lenses were cultured for three days with 1.75 ng/ml TGFβ2 in the absence (A) or presence (B) of 10 mM GSH and then photographed. Lenses cultured with TGFβ alone typically exhibited numerous discrete opacities (A) whereas many lenses cultured with TGFβ and GSH remained transparent (B). The crescent-shaped haziness in each image is an artifact of the light source. C,D: Hematoxylin-eosin stained lens sections. In lenses cultured with TGFβ alone (C), the epithelium typically became multilayered (arrowhead) and contained large anterior subcapsular plaques of cells (arrow). In the lens shown in D, which retained transparency during the four days of culture with 0.3 ng/ml TGFβ2 and GSH, the epithelium remained cuboidal and monolayered as it is in the normal lens. The scale bar represents 120 μm (C) and 60 μm (D). GSH, glutathione.