Table 1 of Gardner, Mol Vis 2009; 15:876-884.
L
or M opsin specific exon 2 PCR primers |
5′-3′ Sequence |
Product size |
Annealing temperature (Ta) °C |
---|---|---|---|
LEx2F | ctggatgatctttgtggtcac | 192 base pairs (bp) | 59 |
LEx2R | cccagcacgaagtagccag | ||
MEx2F | ctggatgatctttgtggtcat | 192 bp | 56 |
MEx2R | cccagcacgaagtagccat | ||
Long
Range PCR primers |
5′- 3′ Sequence | Product size | Ta °C |
LRF | ggctgcactgggggccac | ~7–8 kilobases | 70 |
LRR | aagcaaagcttcccactgtcctgcttagac | ||
Internal
opsin sequencing primers |
5′- 3′ Sequence | ||
Mint1F | tttctcacagctctggaggc | ||
Mint2R | agggagacaggcctaca |