Figure 4. Effects of NTF2 expression on NTF2 and VEGF protein levels. Overexpression of NTF2 by transfection with rAAV2-NTF2 increased NTF2 protein expression but reduced VEGF protein level (B, D). Downregulation of NTF2 by transfection with pSIREN-NTF2 siRNA inhibited NTF2 protein expression but increased VEGF protein level (F, H). The experiments were performed in rat retinal capillary endothelial cells. Representative western blots were shown for the effects of NTF2 overexpression and downregulation on NTF2 (A, E) and VEGF (C, G) preotein expression. Asterisk (*) indicates a significant difference between the groups (p<0.05).