Figure 5. Release of retinal as a function of PA content of SUVs. CRALBP×9-cis-retinal (6 μM) was incubated with NH2OH and SUVs composed of PC and varying amounts of PA (total glycerophospholipid 90 μM). The mole % of PA in the SUVs in indicated
on the abscissa. After incubation for 16 h at room temperature in the dark, retinoids were extracted and quantified by HPLC
analysis. The results shown are representative of two independent repeats of the experiment.