Table 1 of Kim, Mol Vis 2009; 15:833-843. Table 1 of Kim, Mol Vis 2009; 15:833-843.
Table 1. Primers for the RS1 mutation analysis.
| Gene | Usage | Exon | Primer (5′→3′) |
|---|---|---|---|
| RS1 | PCR and sequencing | 1 | F: GGTTAACTTGATGGGGCTCA |
| R: CCCATCCTGTTTTCTGTTGG | |||
| 2 | F: TTCTTCCAGAAGGGGTGTTG | ||
| R: AAGCGATTCTCTTGCCTCAG | |||
| 3 | F: TCAATTTGGCCATTGTAGCA | ||
| R: GGAGAAAACCCGCATTAACA | |||
| 4 | F: TGAACCGTTGAAGACACAGC | ||
| R: AGTGCAGTGGTGTGATCTCG | |||
| 5 | F: TTTCTTGGGAGGTGGAGATG | ||
| R: GCAGATGATCCACTGTGCTG | |||
| 6 | F: GTTCCAGATGTCCCAAGCAT | ||
| R: TGCGAAATATAGCCCTGTCC | |||
| RS1 | Gene dosage | 1 | F: GGGAAGATGTCACGCAAGAT |
| R: AACTGGAAAGCCATCCACAC | |||
| 2 | F: GCCACATTGGGATTATCGTC | ||
| R: TGTTGGGATTACAGGCATGA | |||
| 3 | F: AACCACAGTTGCCTTTGACC | ||
| R: TGTTCCCAATGACTGTTCCA | |||
| 4 | F: CAGTCACCTGGTGCTTGTTG | ||
| R: CGAAGAATACCAGCCCACAT | |||
| 5 | F: TTTCTTGGGAGGTGGAGATG | ||
| R: TGTCCTGGAACTTGGAGAGC | |||
| 6 | F: GTTCCAGATGTCCCAAGCAT | ||
| R: GGTCCGAGTTGCCATAGAAG | |||
| HBB | Gene dosage | 2 | F: TTGGACCCAGAGGTTCTTTG |
| R: GAGCCAGGCCATCACTAAAG | |||
| B2M | Gene dosage | 2 | F: CTCACGTCATCCAGCAGAGA |
| R: AGTGGGGGTGAATTCAGTGT |