Figure 5. Pro370Leu mutant myocilin decreases ATP levels in TM cells. Luciferin/luciferase-based ATP assay indicates that the ATP level in TM with Pro370Leu mutant myocilin (P370L) transfection is 57.7% lower compared to WT myocilin transfection (WT). The ATP levels in non-transfected TM cells (Control) are arbitrarily defined as 100%. The results are expressed as a mean percentage of the ATP levels to the control, error bars represent SE of three repeated experiments done in triplicate. An asterisk indicates a significant difference from non-transfected cells at p<0.05.