Figure 1. Morphology change of TM cells after Pro370Leu mutant myocilin transfection. Phase-contrast light micrographs show healthy looking TM cells in non-transfected (Control), pIRES transfected (Mock), or pIRES-WT myocilin transfected (WT) cultures. However, TM cells demonstrate an unhealthy, degenerative appearance after transfection with pIRES-Pro370Leu mutant myocilin (P370L) for 24 h. Fewer cells are seen in these cultures in part due to cell death and cell detachment. Scale bar=30 μm.