Table 1 of Peng, Mol Vis 2009; 15:810-814. Table 1 of Peng, Mol Vis 2009; 15:810-814.
Table 1. Primers and PCR conditions used to amplify genomic segments of GPR143.
Primer name |
Primer sequence (5′-3′) |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|
| Exon 1-F | AACCTTCCCAACCTTTCTGC | 69 | 698 |
| 1-R | CCTCTCGTCCTCACTCCATC | ||
| Exon 2-F | CAGTGAGCAGGGTTTTTACCA | 66 | 537 |
| 2-R | AACAGACTCCCAGGGTTTGC | ||
| Exon 3-F | GTCTACCCTGCCGTCTCAAG | 66 | 334 |
| 3-R | TGAGCTGCTGTGGATGTTTC | ||
| Exon 4-F | CTCAGCAGCACGAGGAAACT | 67 | 465 |
| 4-R | ACAAACGAGAAAGGCAGAGC | ||
| Exon 5-F | CTTAGGGGTCCTCCCATTTC | 65 | 575 |
| 5-R | TGGCACTGAGCTAACAAACG | ||
| Exon 6-F | ATCCCCATGGTTGCATAAGA | 64 | 738 |
| 6-R | CACATGGTTGGGACATTTCA | ||
| Exon 7-F | GCACCTGGCCCTCTTAGTTT | 67 | 436 |
| 7-R | GAGGCCAAGACAGAGGATTG | ||
| Exon 8-F | TTCAGGCACCCTTGAAGGTA | 66 | 539 |
| 8-R | CCGGGACAAAGAATCCTCTA | ||
| Exon 9-F | GGCTTGTGTCATCCGTTGTA | 61 | 488 |
| 9-R | CCCTTCGGGAAGAAGCTCTA |