Table 1 of Santana, Mol Vis 2009; 15:793-800. Table 1 of Santana, Mol Vis 2009; 15:793-800.
Table 1. Oligonucleotides used as primers for PCR amplification of the CRYAA, CRYGC, and CRYGD, product sizes, and annealing temperatures.
Gene |
Exon |
Strand |
Sequence (5′→3′) |
Product Size (bp) |
T (°C) |
|---|---|---|---|---|---|
| CRYAA | 1 | Sense | CACGCCTTTCCAGAGAAATC | 466 | 59 |
| Antisense | CTCTGCAAGGGGATGAAGTG | ||||
| 2 | Sense | CTTGGTGTGTGGGAGAAGAGG | 377 | 57 | |
| Antisense | TCCCTCTCCCAGGGTTGAAG | ||||
| 3 | Sense | CCCCCTTCTGCAGTCAGT | 989 | 66 | |
| Antisense | GCTTGAGCTCAGGAGAAGGA | ||||
| CRYGC | 1–2 | Sense | ACCAGAGAACAAGGACACAATC | 674 | 62 |
| Antisense | TGGCTTATTCAGGTCTCTGATG | ||||
| 3 | Sense | ATTCCATGCCACAACCTACC | 590 | 62 | |
| Antisense | CCAACGTCTGAGGCTTGTTC | ||||
| CRYGD | 1–2 | Sense | CCCTTTTGTGCGGTTCTTG | 596 | 58 |
| Antisense | TTTGTCCACTCTCAGTTATTGTGAC | ||||
| 3 | Sense | TGTGCTCGGTAATGAGGAG | 700 | 62 | |
| Antisense | AGGCCAGAGAATCAAATGAG |