Figure 7 of Rada, Mol Vis 2009; 15:778-792.


Figure 7. ATH protein accumulation in posterior chick ocular tissues from eyes in various growth states. Retina–RPE, choroid, sclera, and suprachoroidal fluid were harvested from the posterior poles of 10-day-old untreated, normal eyes (N), following 10 days of form deprivation (FD), and following 1, 4, and 7 days of unrestricted vision with prior form deprivation for 10 days (1 day, 4 day, and 7 day recovery, respectively) together with tissues from contralateral control eyes. Total protein (8 μg) from each tissue extract was separated by SDS–PAGE, and ATH protein expression was detected by western blot analysis. The 11.5 kDa band from each sample was quantified by densitometry using NIH Image v. 1.63. Bars represent the integrated optical density (IOD) of ATH bands on western blots from treated eyes minus IOD of contralateral controls (mean±SEM). A: ATH protein expression was measured in the posterior retina/RPE. B: ATH protein expression was measured in the posterior choroid. A large increase in ATH was detected in choroids following 4 days of recovery as compared with ATH levels in choroids of contralateral controls. C: ATH protein expression was measured in the posterior sclera. D: ATH protein accumulation was measured in aliquots of suprachoroidal fluid isolated from the posterior poles of enucleated eyes. E: ATH protein expression was measured in choroids following 4 days of recovery and in contralateral control eyes (n=6 additional pairs of control and treated eyes). F: Representative western blots are shown of ATH protein in retina/RPE (Ret), choroid (Chor), sclera (Scl), and suprachoroidal fluid (Supra) in treated (R) and control (C) eyes following 4 days of recovery. Data represent the mean±SEM for n=3 birds (3 pairs of control and treated eyes for figures A-D and n=6 pairs for figure E) in each group *p<0.05. Comparisons between recovering and contralateral control eyes were made using the Wilcoxon signed-rank test for paired data.