Figure 6. Specificity of anti-ATH on
sections of chick ocular tissues. Fixed cryostat sections of chick
sclera and choroid were incubated with A) monoclonal anti-ATH
antibody (ATH positive), with B) anti-ATH antibody that was
preincubated with 2 μM recombinant chick ATH (ATH protein block), or
with C) nonimmune mouse IgG (IgG control) followed by
incubation in anti-mouse IgG conjugated to AlexaFluor 568 (red). No
specific immunoreactivity is observed in fibrous sclera (FS) and
choroid when antibodies were preincubated with ATH, indicating the
specificity of the anti-ATH antibodies for ATH in chick ocular tissues,
cartilaginous sclera is abbreviated as CS.