Figure 5 of Rada, Mol Vis 2009; 15:778-792.


Figure 5. Distribution of ATH in the sclera and choroid of control and recovering chick eyes. Monoclonal antibodies specific for ATH were used together with AlexaFluor 488-conjugated rabbit anti-mouse immunoglobulin to localize ATH in sclera and choroid of control and recovering eyes. A: Intense ATH immunolabeling is detected in the fibrous sclera (FS) and in perivascular and extravascular regions of the choroid. ATH was absent in the cartilaginous scleral layer (CS). B: IgG control section of the sclera and choroid of a control eye, where nonimmune mouse IgG was used in the first incubation, followed by incubation in AlexaFluor 488-conjugated rabbit anti-mouse immunoglobulin. C: ATH immunolabelling in the sclera of a recovering chick eye is shown in a region artifactually separated from the choroid. ATH can be seen localized in the outer fibrous sclera (FS) as well as in the thin inner fibrous sclera on the choroidal side of the sclera. D: ATH immunolabeling in the choroid of a recovering chick eye is shown. ATH can be seen throughout the stroma of the markedly expanded choroid and on the choroidal side of Bruch’s membrane (arrow), but is absent in the RPE. Nuclei were stained with DAPI (blue). Choriocapillaris is abbreviated CC. Scale bar (A–D) represents 50 μm.