Figure 3 of Rada, Mol Vis 2009; 15:778-792.


Figure 3. Localization of Avian Thymic Hormone (ATH) labeling in posterior ocular tissues of control and recovering chick eyes. Monoclonal antibodies specific for ATH (obtained from Michael Henzel, University of Missouri, Columbia, MO) were used together with AlexaFluor 488-conjugated rabbit anti-mouse immunoglobulin to localize ATH in chick ocular tissues. A: ATH immunolabeling of a control eye demonstrated intense labeling in the fibrous scleral layer (FS) and the choroid. Additionally, ATH was detected in a subpopulation of cell bodies located in the inner nuclear layer (INL) and in two sublaminae of the inner plexiform layer, most likely representing the synaptic processes of ATH-positive cells of the INL. B: IgG control section of a control eye is shown where non-immune mouse IgG was used in the first incubation, followed by incubation in AlexaFluor 488-conjugated rabbit anti-mouse immunoglobulin. Fluorescence of lipid droplets in the photoreceptor inner segments (*) and in the nerve fiber layer is nonspecific. Nuclei were stained with DAPI (blue). C: ATH distribution in a recovering eye was similar to that of controls, with specific immunolabeling in the fibrous sclera, as well as throughout the markedly thickened choroid, cells of the INL and sublaminae of the inner plexiform layer (IPL). Vertical bars indicate thickness of choroid layer in control (3A) and recovering (3C) eyes. Abbreviations: cartilaginous sclera (CS), outer nuclear layer (ONL), ganglion cell layer (GCL). Scale bar (A–C) equals 100 μm.