Figure 1. Real time RT–PCR quantification of ATH in chick ocular tissues. Steady-state levels of ATH mRNA were measured in the retina–RPE, choroid, sclera, and extraocular muscle using chick gene-specific primers (Table 1). A: The average mean normalized expression (MNE) of ATH was calculated in each ocular tissue of three untreated 10 day old chicks. All reactions were run in triplicate and normalized to the reference gene, GAPDH. B: Shown are ethidium bromide gels of real-time PCR products of ATH and GAPDH in chick extraocular muscle over temperatures ranging 66–55 °C. Mr: PhiX174RF DNA/HaeIII molecular weight ladder, Lane 1: 66.0 °C, Lane 2: 65.2 °C, Lane 3: 63.9 °C, Lane 4: 61.8 °C, Lane 5: 59.0 °C, Lane 6: 57.2 °C, Lane 7: 55.8 °C, Lane 8: 55.0 °C.