Table 3 of Kirwan, Mol Vis 2009; 15:76-88.

Table 3. Validation of the microarray expression patterns of POAG versus normal LC cells by real time quantitative PCR.

Gene Microarray analysis Real time PCR
Upregulated in POAG Mean fold change (n=2) Mean fold change (n=3)
periostin 8 35 (*p=0.008)
cartilage linking protein-1 3.5 240 (*p=0.003)
dystrophin 4 3.2 (*p=0.0005)
sulfatase-1 2 8 (*p=0.006)
rho GDP dissociation inhibitor beta 2 4.5 (*p=0.02)
thrombospondin-1 1.7 1.7 (*p=0.02)
BMP-1 1.6 2 (*p=0.02)



Downregulated in POAG Mean fold change (n=2) Mean fold change (n=3)
neuritin-1 2 60 (*p=002)
prostaglandin D2 synthase 3.2 22 (*p=0.02)

Columns show the gene name, the fold expression change (converted from signal log ratio (SLR) using the formula: fold change=2(signal log ratio)) determined by microarray and the corresponding expression change determined by real time PCR. Results for real time PCR are shown in bold and are expressed as the mean of three separate experiments (n=3) with the calculated p value, where *p<0.05 was considered to be statistically significant.

Kirwan, Mol Vis 2009; 15:76-88. http://www.molvis.org/molvis/v15/a8

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