Molecular Vision: Abouzeid, Mol Vis 2009; 15:771-777. Table 1

Table 1. Primers and PCR conditions.

Region	Primer	Sequence 5′-3′	Amplicon [bp]	Annealing [°C]
promoter	PromF	CTGGACCCACGCCAGGTTTC	340	61
promoter	PromR	GTTTTGGGCGGCATGACGCCTT	340	61
RB exon 1	1F	CCGGTTTTTCTCAGGGGACGTTG	340	56.4
RB exon 1	1R	TTGGCCCCGCCCTACGCACAC	340	56.4
RB exon 2	2F	CTATTGAAACAAGTATGTACTG	331	54.3
RB exon 2	2R	GGGTAATGGAATTATTATTAGC	331	54.3
RB exon 3	3F	CAGTTTTAACATAGTATCCAG	281	52.8
RB exon 3	3R	AGCATTTCTCACTAATTCAC	281	52.8
RB exon 4	4F	GTAGTGATTTGATGTAGAGC	305	55
RB exon 4	4R	CCCAGAATCTAATTGTGAAC	305	55
RB exon 5	5F	GCATGAGAAAACTACTATGAC	194	54.3
RB exon 5	5R	CTAACCCTAACTATCAAGATG	194	54.3
RB exon 6	6F	CACCCAAAAGATATATCTGG	222	54.3
RB exon 6	6R	ATTTAGTCCAAAGGAATGCC	222	54.3
RB exon 7	7F	CCTGCGATTTTCTCTCATAC	256	55
RB exon 7	7R	ATGTTTGGTACCCACTAGAC	256	55
RB exon 8	8F	AGTAGTAGAATGTTACCAAG	380	50.8
RB exon 8	8R	TACTGCAAAAGAGTTAGCAC	380	50.8
RB exon 9	9F	TGCATTGTTCAAGAGTCAAG	222	56
RB exon 9	9R	AGTTAGACAATTATCCTCCC	222	56
RB exon 10	10F	TCTTTAATGAAATCTGTGCC	291	56
RB exon 10	10R	GATATCTAAAGGTCACTAAG	291	56
RB exon 11	11F	GAGACAACAGAAGCATTATAC	245	54.2
RB exon 11	11R	CGTGAACAAATCTGAAACAC	245	54.2
RB exon 12	12F	GGCAGTGTATTTGAAGATAC	310	52.5
RB exon 12	12R	AACTACATGTTAGATAGGAG	310	52.5
RB exon 13	13F	CTTATGTTCAGTAGTTGTGG	342	54.3
RB exon 13	13R	TATACGAACTGGAAAGATGC	342	54.3
RB exon 14	14F	GTGATTTTCTAAAATAGCAGG	212	58.9
RB exon 14	14R	TGCCTTGACCTCCTGATCTG	212	58.9
RB exon 15+16	15/16F	CAATGCTGACACAAATAAGG	366	55
RB exon 15+16	15/16R	AGCATTCCTTCTCCTTAACC	366	55
RB exon 17	17F	AAAAATACCTAGCTCAAGGG	339	56
RB exon 17	17R	TGTTAAGAAACACCTCTCAC	339	56
RB exon 18	18F	TGTACCTGGGAAAATTATGC	340	56.4
RB exon 18	18R	CTTTATTTGGGTCATGTACC	340	56.4
RB exon 19	19F	ATAATCTGTGATTCTTAGCC	273	56
RB exon 19	19R	AAGAAACATGATTTGAACCC	273	56
RB exon 20	20F	AAAGAGTGGTAGAAAAGAGG	335	56.4
RB exon 20	20R	CAGTTAACAAGTAAGTAGGG	335	56.4
RB exon 21	21F	AAACCTTTCTTTTTTGAGGC	328	54
RB exon 21	21R	TACATAATAAGGTCAGACAG	328	54
RB exon 22	22F	TAATATGTGCTTCTTACCAGTC	313	56
RB exon 22	22R	TTTAATGTTTTGGTGGACCC	313	56
RB exon 23	23F	ATCTAATGTAATGGGTCCAC	287	54.2
RB exon 23	23R	CTTGGATCAAAATAATCCCC	287	54.2
RB exon 24	24F	GAATATAGTTTGTCAGTGGTTC	273	52 53
RB exon 24	24R	GTGTTTGAATAACTGCATTTGG	273	52 53
RB exon 25	25F	GGTTGCTAACTATGAAACAC	297	54.2 55
RB exon 25	25R	AGAAATTGGTATAAGCCAGG	297	54.2 55
RB exon 26	26F	AGTAAGTCATCGAAAGCATC	209	52.8
	26R	AACGAAAAGACTTCTTGCAG	209	52.8
RB exon 27	27F	CGCCATCAGTTTGACATGAG	237	54.2