Figure 8 of Xiao, Mol Vis 2009; 15:747-761.


Figure 8. Quantitative real-time polymerase chain reaction analyses of mRNA expression of senescence-related genes after normalization to GAPDH in the corneal endothelial cells of SAM R1 and P8 strains at various ages. A: Quantitative comparison of the fold difference in the expression of p16INK4a mRNA is shown at various ages in each experimental group. The results indicate that the expression of p16INK4a was significantly increased from six months old to 12 months old in the SAM R1 strain experimental group (p=0.027 and p=0.000, respectively). In the SAM P8 strain experimental group, a significant increase was observed at six months (p=0.000 when compared to the one-month-old control group) and a significant decrease was observed at 12 months (p=0.000 when compared to the value at six months), although comparison between the 12-month- and 1-month-old values still resulted in a statistically significant increase (p=0.008). B: Quantitative comparison of the fold difference in the expression of p19ARF mRNA is shown at various ages. The results indicate that the expression of p19ARF mRNA decreased significantly at six months in the SAM R1 strain experimental group (p=0.000). C: Quantitative comparison of the fold difference in the expression of p21WAF1/CIP1 mRNA is shown. The results indicated that the expression of p21WAF1/CIP1 was significantly higher at 12 months in the SAM P8 strain experimental group (p=0.007). D: Quantitative comparison of the fold difference in the expression of p53 mRNA is shown. The results indicate that the expression of p53 decreased significantly at six months of age in the SAM R1 strain experimental group, although no significant difference was observed at 12 months of age (when compared to the value at one month; p=0.000, p=0.927, respectively). However, the expression of p53 increased significantly at 12 months of age in the SAM P8 strain experimental group (p=0.000). There were three individual corneas used in each experimental group, and each cDNA sample had been detected three times. An asterisk marks a statistically significant difference (p<0.05).