Figure 5. Staining of senescence-associated β-galactosidase activity in corneal endothelial cells of the SAM R1 and the SAM P8 strains at various ages. The positive stainings are shown in blue. The staining of the SAM R1 strain at one month, six months, and 12 months are shown (A, C, and E, respectively). The staining of the SAM P8 strain at one month, six months, and 12 months are also displayed (B, D,and F, respectively). There were few to no positive stainings for SA-β-Gal activity in panels A and B while there were more multifocal and intense positive stainings in panels C, D, E, and F. Magnification: 400X.