Table 1 of Narendra, Mol Vis 2009; 15:731-736.
Table 1. PCR conditions and primers used to amplify CFHR5 in this study.
Exon |
Primers (3′-5′) |
Annealing temperature (°C) |
Buffer pH |
MgCl2 (mM) |
% DMSO |
Fragment (bp) |
---|---|---|---|---|---|---|
1 | F: GCAGATTTAAAGCAACACCACC | 60 | 8.4 | 1 | 0 | 242 |
R: CCCCTTCAAATTATCCTCAGC | ||||||
2 | F: CTAGTGATTCATCGATGTAGCTC | 52 | 8.4 | 1 | 10 | 370 |
R: TTTCCAGCTCCTCTGGTCATTGC | ||||||
3 | F: TGATGTCAGTTTTCAAAGTTTTCC | 54 | 8.4 | 1 | 0 | 313 |
R: TAATTAGCAAAACTGAGAGAGTGG | ||||||
4 | F: GCACACATTAAATTTGTTTCTGCA | 54 | 8.4 | 1.5 | 0 | 345 |
R: TACCACTTTGTCAGATTATAGATG | ||||||
5 | F: TAGTAACTCCACATTTTTCTATAC | 50 | 8.4 | 1.5 | 0 | 301 |
R: TAAGTGCAAAGTAATAGTAACTGT | ||||||
6 | F: AATATTTTCAGAGTAAGCACTCAT | 50 | 8.4 | 1.5 | 0 | 288 |
R: ACACTTTATACAATCCCAATCAAA | ||||||
7 | F: GTGATGTTGCTTAAAAGCATCAAAC | 54 | 8.6 | 1.5 | 0 | 325 |
R: CTTGTAAAGAAGCAACAAGATCAAC | ||||||
8 | F: CCATTTTCCTGAAACACTACCCTA | 52 | 8.4 | 1.5 | 0 | 377 |
R: TTGGGGTACAGTGCAACAGATTAG | ||||||
9 | F: AATTATTTGAATTTCCAGACACCT | 50 | 8.4 | 1.5 | 0 | 375 |
R: GGGTTATTCTATGAAATTAGTCCA | ||||||
10 | F: GCAATTTCACTATTCTATGAAAGG | 52 | 8.4 | 1.5 | 0 | 348 |
R: ACAGAATTGGCTACATAATGGCT |