Table 1 of Narendra, Mol Vis 2009; 15:731-736. Table 1 of Narendra, Mol Vis 2009; 15:731-736.
Table 1. PCR conditions and primers used to amplify CFHR5 in this study.
Exon |
Primers (3′-5′) |
Annealing temperature (°C) |
Buffer pH |
MgCl2 (mM) |
% DMSO |
Fragment (bp) |
|---|---|---|---|---|---|---|
| 1 | F: GCAGATTTAAAGCAACACCACC | 60 | 8.4 | 1 | 0 | 242 |
| R: CCCCTTCAAATTATCCTCAGC | ||||||
| 2 | F: CTAGTGATTCATCGATGTAGCTC | 52 | 8.4 | 1 | 10 | 370 |
| R: TTTCCAGCTCCTCTGGTCATTGC | ||||||
| 3 | F: TGATGTCAGTTTTCAAAGTTTTCC | 54 | 8.4 | 1 | 0 | 313 |
| R: TAATTAGCAAAACTGAGAGAGTGG | ||||||
| 4 | F: GCACACATTAAATTTGTTTCTGCA | 54 | 8.4 | 1.5 | 0 | 345 |
| R: TACCACTTTGTCAGATTATAGATG | ||||||
| 5 | F: TAGTAACTCCACATTTTTCTATAC | 50 | 8.4 | 1.5 | 0 | 301 |
| R: TAAGTGCAAAGTAATAGTAACTGT | ||||||
| 6 | F: AATATTTTCAGAGTAAGCACTCAT | 50 | 8.4 | 1.5 | 0 | 288 |
| R: ACACTTTATACAATCCCAATCAAA | ||||||
| 7 | F: GTGATGTTGCTTAAAAGCATCAAAC | 54 | 8.6 | 1.5 | 0 | 325 |
| R: CTTGTAAAGAAGCAACAAGATCAAC | ||||||
| 8 | F: CCATTTTCCTGAAACACTACCCTA | 52 | 8.4 | 1.5 | 0 | 377 |
| R: TTGGGGTACAGTGCAACAGATTAG | ||||||
| 9 | F: AATTATTTGAATTTCCAGACACCT | 50 | 8.4 | 1.5 | 0 | 375 |
| R: GGGTTATTCTATGAAATTAGTCCA | ||||||
| 10 | F: GCAATTTCACTATTCTATGAAAGG | 52 | 8.4 | 1.5 | 0 | 348 |
| R: ACAGAATTGGCTACATAATGGCT |