Figure 3. AND-34 expression in mouse tissues. A: Western blot analysis of wild type mouse tissues is shown. AND-34 was immunoprecipitated directly from lysates of mouse organs with polyclonal anti-AND-34 antibody whereas p130CAS was detected directly from whole cell lysates with a monoclonal anti-p130CAS antibody that is also able to detect HEF1. B: In situ hybridization for AND-34 transcripts from lymph nodes and spleens of AND-34^+/+ is displayed. The tissues were probed with sense or antisense hybridization probes as shown in dark-field microscopy. With dark-field illumination, the positive hybridization signal of the silver grains in the autoradiographic emulsion appears as white dots over a black background. C: In situ hybridization for AND-34 transcripts in the lungs of AND-34^+/+ mice is shown in bright-field microscopy. With bright-field illumination, the positive hybridization signal appears as black dots. Hybridization was detected in a subset of alveolar cells with the morphology of lung fibroblasts. Arrows indicate a strong hybridization signal.