Figure 2. Exons 4 and 5 of AND-34^−/− mice lack AND-34 protein. Immunoblots of whole cell lysates from splenic lymphocytes from wild type (+/+) and knockout (−/−) mice were performed with two different polyclonal anti-AND-34 antisera. As positive and negative controls, lysates of 293T cells transfected with an AND-34 expression plasmid (pAND) or vector alone (pcDNA) were also included. A: AND-34^−/− mice in which exon 2 had been targeted were assessed with antiserum to peptide cYEKQLKPFSKLLHEGREST. The presence of residual truncated AND-34 protein product is noted in knockout splenic lymphocytes. B: AND-34^−/− mice in which exons 4 and 5 had been targeted were examined with antiserum to peptide KSPLAERRTDAYQDVSIc. The AND-34 protein is absent in knockout splenic lymphocytes. A non-specific band (N.S.) is detected with this antiserum.