Figure 4 of Dot, Mol Vis 2009; 15:670-684.

Figure 4. Representative phase contrast image and immunohistochemistry four months after photocoagulation in 1-year-old mice (group IV). A: Phase contrast image demonstrates a capsular and pigmented cell reaction that closes the initial retinal pigment epithelium (rpe) interruption and surrounds the choroidal neovascularization (CNV) reaction. Overlying the lesion, photoreceptor outer segments are markedly affected. B: Immunodetection of von Willebrand factor (green) reflects the CNV presence at least four months after photocoagulation limited by the surrounding new RPE. The new vessels do not infiltrate the retina. Astrocytes and activated Müller cells were detected with specific antibodies for glial fibrillary acidic protein (GFAP, red). Nuclei were counterstained with 4’,6-Diamidino-2-Phenyl-Indole (DAPI, blue). Scale bar in A represents 100 µm in both panels. Abbreviations: choroid (ch), ganglion cell layer (gcl), inner nuclear layer (inl), outer nuclear layer (onl), rod outer segments (ros), retinal pigment epithelium (rpe), sclera (scl).