Figure 2. : Flat mounts showing choroidal neovascularization in mice from groups III and IV. Images of flat mounts of 10–12-week-old
mice (group III; A, B) and 1-year-old mice (group IV; C, D), after immunolabeling done with lectin from Bandeiraea simplicifolia fluorescein isothiocyanate (FITC)-labeled (green),
anti-glial fibrillary acidic protein (GFAP) antibodies (red) and 4’,6-Diamidino-2-Phenyl-Indole (DAPI; blue). A: At day 7 after photocoagulation (PC), new vessels surround the GFAP+cells activated in the center of the laser beam. The
size of the neovascular reaction measures 399 µm in its largest diameter (initial laser beam of 50 µm). B: Six months after PC, new vessels are more delineated (arrow) around the activated GFAP+cells. C: At day 7 after PC, new vascular reaction extends like a pool. Size of the largest diameter measures 421 µm. D: Eight months after PC, Müller cells are still activated in the center of the laser beam. New vessels are dilated and appear
fully developed (arrow). Scale bar (A) represents 100 µm for each panel.