Figure 10. MCP-1 expression after photocoagulation in RPE-choroid complex. A: The amount of monocyte chemotactic protein-1 (MCP-1) transcripts was compared with 18S transcripts (y-axis) expressed in the same samples. These data are from four different eyes. Values in histogram are means±SEM B,C: Representative samples of PCR fragments (individual ratio of MCP-1:18S closest to the corresponding mean) for 10–12-week-old mice (B) and 1-year-old mice (C) were analyzed by 3% agarose gel electrophoresis and visualized by ethidium bromide staining under ultraviolet light. Prior to laser treatment MCP-1 mRNA expression was very low. After photocoagulation, it is strongly expressed in the retinal pigment epithelium-choroid complex; its level of expression is higher and observed for a longer period of time in old mice.