Figure 1. Cellular reactions after photocoagulation in 10–12-week-old mice (group III). Representative phase contrast images and immunohistochemistry of sections from eyes at day 7 (A, C), and month 6 (B, D) following photocoagulation. A: Phase contrast image demonstrates a localized disruption of retinal pigmented epithelium (rpe) and choroid (ch) in the center of the laser beam (arrows). B: Phase contrast image demonstrates the formation of a mound of poorly defined cells surrounded by lightly pigmented retinal pigmented epithelium (rpe) structures (arrow). C: Immunohistofluorescence analysis was performed with specific antibodies for von Willebrand (green) and glial fibrillary acidic protein (GFAP, red). Nuclei were counterstained with 4’,6-Diamidino-2-Phenyl-Indole (DAPI, blue). This section also illustrates a marked activation of GFAP-positive cells (astrocytes and Müller cells) in the center of the laser beam and von Willebrand reaction in the choroidal site (thin arrows). D: Six months after photocoagulation, a localized von Willebrand reaction is still evident at the level of the choroid (thin arrows). Abbreviations: ganglion cell layer (gcl), inner nuclear layer (inl), inner plexiform layer (ipl), outer nuclear layer (onl), sclera (scl). Scale bar (A) represents 100 µm for each panel.