Figure 5. Colocalization of BDNF with GFAP by double immunofluorescence. A: Schematic drawing shows the coronal section through the level of the superior colliculus (SC; bregma −3.40 mm) in mice. The boxed area is the region of the superficial layer in the contralateral SC. Representative photographs show brain-derived neurotrophic factor (BDNF; B and C) and glial fibrillary acid protein (GFAP; D and E) immunostaining, and BDNF/GFAP (F and G) double-immunostaining of the contralateral SC at 30 days after intravitreal N-methyl-D-aspartate (NMDA) injection in mice. Some BDNF-expressing cells (B and C, green) were colocalized with GFAP-positive astroglial cells (D and E, red), as indicated by the yellow color in F (merge of B and D) and G (merge of C and E). The scale bars represents 50 µm (B, D, and F) or 10 µm (C, E, and G).