Figure 4. T cell distribution in the ocular surface of Aire-deficient mice. Assemblage of low magnification images showing anatomic locations analyzed in Aire^−/− (A) and Aire^+/− (B) mice. Cj=conjunctiva; Lm=limbus; Co=cornea (paracentral and central). Immunohistochemistry of CD4⁺ (C,D) and CD8⁺ T (E,F) cells demonstrates both epithelial (white arrow) and stromal (black arrow) infiltration of the ocular surface in Aire^−/−. Aire^+/− controls exhibited no cell-mediated inflammation at the locations analyzed. Bar, 100 μm. G: Time course of CD4⁺ and CD8⁺ cell density in Aire^−/− and Aire^+/−. Data are shown as mean±SD. An asterisk indicates that p<0.05, CD4⁺Aire^+/− versus CD4⁺Aire^−/−. The symbol, †, indicates that p<0.05, CD8⁺Aire^+/− versus CD8⁺Aire^−/−.