Figure 3. Expression of squamous cell biomarker, SPRR1B. A: Immunofluorescence showed increased SPRR1B staining (green) in Aire^−/− at all time points with no staining in age-matched Aire^+/− controls. Bar, 100 μm. B: Quantification of SPRR1B immuno-intensity over time. Data are shown as mean±SD. An asterisk in indicates that p<0.05, Aire^+/− versus Aire^−/− at each time point.