Figure 2. Time course of goblet cell density in Aire^+/− versus Aire^−/− mice. A: Periodic acid–Schiff staining revealed abundant goblet cells (arrow) in Aire^+/− at all time points (representative example shown) and in Aire^−/− at four weeks of age. A gradual decrease in goblet cell density was observed in the conjunctiva of Aire^−/− mice over time. Bar, 100 μm. B: Time course of goblet cell density is shown as mean±SD. An asterisk indicates that p<0.05, Aire^+/− versus Aire^−/− at each time point.