Figure 3. Two dimensional analysis of myocilin expression. Myocilin isoforms in cell lysate (upper panel) and culture medium (lower panel) from HTM cells transduced with Ad-myocilin-FLAG were first separated by their isoelectric points and then resolved according to their molecular weights. Myocilin detection was performed by western blot analysis using anti-FLAG antibody. This experiment was performed two times, and representative gels are shown.