Figure 3. In situ hybridization analysis of miR-182 in eyes of normal embryonic mice. Eyes of embryonic mice on E14.5 (A-D) and E16.5 (E-H) were analyzed. Matched LNA probes specific for miR-182 (miR182LNA, upper panels) or a control probe containing a single-base mismatch (miR182LNA/MM, lower panels) was used for the hybridization. The unhybridized LNA probes were washed out at 25 °C Note that the matched probe produced the strongest signals in the inner retina (magnified upper panels).