Figure 2. In situ hybridization analysis of miR-182 expression in P3 eyes. A matched LNA probe specific for miR-182 (LNA) or a control probe containing a single-base mismatch (LNA/MM) was used for the hybridization. The probe sequences are shown. The unhybridized LNA probes were washed out at 25 °C (A-F), 35 °C (G-L), or 45 °C (M-R). Magnifications are either ×16 (A, D, G, J, M, P) or ×400 (B, C, E, F, H, I, K, L, N, O, Q, R). The strongest staining was observed at 25 °C and the signals became fainter and eventually disappeared as the washing temperature increased.