Figure 9. Inflammatory condition improved the penetration of anti-CD45 antibody in the mouse cornea. Leukocytes in the corneal stroma were labeled by rat anti-mouse CD45 antibody (red) 24 h after intrastromal LPS injection, and the signals were stronger on the upper side near the cutting edge than that on the down side. Inflammation caused by LPS improved the penetration of the anti-CD45 antibody that labeled leukocytes in the corneal stroma, but CD45 labeling was limited in the corneal epithelium and anterior stroma. The images of Z-stack clearly showed that leukocytes located in the epithelium and anterior stroma were labeled by CD45 antibody as shown in the XZ and YZ planes of the cut view of Z-stack CLSM images. In A the image of photo-section 29 is shown. In B the image at photo-section 32 (3 µm deeper from photo-section 29) is shown. White arrows show leukocytes that were not labeled by anti-CD45 antibody. Scale bar: 10 µm.