Figure 4. Distribution of F-actin in the mouse corneal cells and formation of filopodia and lamellipodia during healing of epithelium debridement. In the superficial epithelium, F-actin (red) is homogeneously distributed within whole cell body (A). However, in the corneal basal and wing cell layer, the microfilament networks are mostly gathered in the cytoplasmic circumference (B). In the corneal stroma, phalloidin labeling displays large and flat dendritic cell body of keratocyte (C,D). In the corneal endothelium, the distribution of F-actin gathered in the circumferential cytoplasm similar to that of the basal epithelial cell layer (E).The squamous epithelial cells at the leading edge altered the polygonal cell shape into spindle-shape and scallop-shape that oriented onto the substratum with scallop-shaped lamellipodia and filopodia. Polymorphonuclear neutrophils invading the stroma following corneal epithelium debridement were readily visible (F). Scale bar: 10 μm.