Figure 3. Distribution of β-tubulin in mouse cornea. β-Tubulin (red) exhibits an extensive arrangement in the epithelium, and the de-convoluted cytoskeleton fills the whole cell body (A). In the corneal stromal cells, the microtubule extended from the nucleus to the cell membrane (B). In the corneal endothelium, the convoluted microtubules twine around the nuclei, and very few microtubules extended to cell membrane (C). Following the conventional whole mount immunostaining, only the corneal endothelium was labeled by anti-β-tubulin (D). Scale bar: 10 μm.