Figure 3. Changes in actin filament staining at the iridocorneal angle of the RGS2^−/− mice. Cryosections of the eye anterior chamber including the iridocorneal region derived from the RGS2^−/− and wild-type mice were labeled for filamentous actin with rhodamin-phalloidin, and fluorescence images were recorded. Actin filament staining (bright red fluorescence) in the ciliary muscle (CM) and trabecular meshwork (TM) of the RGS2^−/− mice (C and D) was found to be more intense relative to the wild-type mice (A and B), with much stronger staining in the ciliary body (CB) as opposed to the TM. Panels E-H show images of the same specimens stained for the actin filaments (A-D), but recorded under bright-field settings. Abbreviations: retina (RT); Schlemm’s canal (SC); sclera (SCL). Scale bar represents 50 µm.