Table 1 of Kozulin, Mol Vis 2009; 15:45-59.

Table 1. QRT–PCR primers

NCBI RefSeq Gene Forward primer (5′-3′) Reverse primer (5′-3′)
NM_001080448 Eph-A6 TTTTTCTCCCAAGCCATTCA ATGCCCAGTCCTTCCTTACC
NM_004428 ephrin-A1 GGTGACTGTCAGTGGCAAAA GCACTGTGACCGATGCTATG
NM_001405 ephrin-A2 GCCTGCGACTGAAGGTGTA CGGGCTGCTACACGAGTTAT
NM_004952 ephrin-A3 ACTCTCCCCCAGTTCACCAT GTCCCGCTGATGCTCTTCT
NM_005227 ephrin-A4 ACTACATCTCGGTGCCCACT CTGATGTGCCACTCTCTCCA
NM_001962 ephrin-A5 TTCATGATCGTGTTTTCGATG GCTGGGTATCCTTGGTGTTT
NM_002046 GAPDH TGCACCACCAACTGCTTAGC GGCATGGACTGTGGTCATGAG

QRT–PCR primers were designed to verify gene expression of Eph-A6 and its ligands ephrin-A1 and -A4, as well as ephrin-A2, -A3, and -A5. These genes were amplified using a SYBR GreenER QRT–PCR kit (Invitrogen) and amplification was normalized relative to a GAPDH reference gene. QRT–PCR reactions were done in triplicate and repeated using cDNA from three different specimens.

Kozulin, Mol Vis 2009; 15:45-59. http://www.molvis.org/molvis/v15/a5

©2009 Molecular Vision http://www.molvis.org/molvis/

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