Table 3 of Oshikawa, Mol Vis 2009; 15:482-494.

Table 3. Primers for construction of expression vectors.

Gene Primer name Sequence (5′-3′)
ARSA-FLAG 370fw-HindIII taaagcttATGTCCATGGGGGCACCGCG
1896rv-KpnI atggtaccGGCATGGGGATCTGGGCAAT
ARSI-FLAG 580fw-EcoRI ctgaattccaccATGCACACCCTCACTGGCTTC
2287rv-BglII ttagatctccGATCCGTTGGGACATTAGCCT
ARSICΔ46-FLAG 580fw-EcoRI ctgaattccaccATGCACACCCTCACTGGCTTC
2211rv-BglII ttagatctGCCCAGGGCCCCCAAGCA
SUMF1-FLAG 15fw-HindIII tcaagcttCGCGGGACAACATGGCTG
1147rv-KpnI ttggtaccGTCCATGGTGGGCAGGCG
SUMF1-myc 20fw-HindIII ataagcttGACAACATGGCTGCGCCC
1147rv-SacII ttccgcggGTCCATGGTGGGCAGGCG
S1ARSI-myc SUMF1fw CGCCTGCCCACCATGGAC
SUMF1rv-XbaI cttctagaCCCTGCCGCTCCACACAG
ARSIfw-XbaI cctctagaCCTCCCCACATCATCTTCA
ARSIrv-SacII atccgcggGATCCGTTGGGACATTAGCCT
S1ARSICΔ46-FLAG SUMF1–20fw-EcoRI atgaattcGACAACATGGCTGCGCCC
ARSI-2211rv-BglII ttagatctGCCCAGGGCCCCCAAGCA

In the "Sequence" column, uppercase letters represent a cDNA sequence and lowercase letters denote a restriction site. Bold underlining indicates an initiation codon. The coding region of each gene was amplified using the primer set. The amplified product was cut with the corresponding restriction enzyme and was then cloned into pFLAG-CMV-5 or pcDNA3.1/myc-His.

Oshikawa, Mol Vis 2009; 15:482-494. http://www.molvis.org/molvis/v15/a49

©2009 Molecular Vision http://www.molvis.org/molvis/

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